SAMHD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SAMHD1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

65

Kat-Nr. : 12586-1-AP

Synonyme

DCIP, HDDC1, Monocyte protein 5, MOP 5, MOP5, SAM domain and HD domain 1, SAMHD1, SBBI88

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Galerie der Validierungsdaten

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  WB analysis using 12586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12586-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12586-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12586-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12586-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12586-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12586-1-AP

  IP Result of anti-SAMHD1 (IP:12586-1-AP, 3ug; Detection:12586-1-AP 1:300) with K-562 cells lysate 2400ug.

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  IHC staining of human lung cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12586-1-AP (SAMHD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12586-1-AP (SAMHD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 12586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAMHD1 antibody (12586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of THP-1 using 12586-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.2 ug Anti-Human SAMHD1 (12586-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), or 0.2 ug Control Antibody (black). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

• 

  FC experiment of K-562 using 12586-1-AP

  1X10^6 K-562 cells were stained with 0.2ug SAMHD1 antibody (12586-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Herzgewebe, humanes Hirngewebe, K-562-Zellen, L02-Zellen, Mausherzgewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: THP-1-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 14 publications below
• WB: See 42 publications below
• IHC: See 9 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• FC: See 11 publications below

Produktinformation

12586-1-AP bindet in WB, IP, IHC, IF, FC, ELISA SAMHD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAMHD1 fusion protein Ag3287
• Vollständiger Name: SAM domain and HD domain 1
• Berechnetes Molekulargewicht: 626 aa, 72 kDa
• Beobachtetes Molekulargewicht: 64-72 kDa
• GenBank-Zugangsnummer: BC036450
• Gene symbol: SAMHD1
• Gene ID (NCBI): 25939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.

Publikationen

Species	Application	Title
human	IF	Nat Genet; Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response.; Authors - Rice Gillian I GI
human	IF	Nat Microbiol; Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97.; Authors - Ramona Businger
human	WB,IHC	Nat Med; PMID: 27991919; Authors - Constanze Schneider
human	WB,IHC	Nat Med; PMID: 28067901; Authors - Nikolas Herold
human	WB, IHC, IF	Nat Med; SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells.; Authors - Baldauf Hanna-Mari HM ; KD Validated
monkey	WB	Immunity; Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells.; Authors - Nina Calantone

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