SAP 97 Polyklonaler Antikörper

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• KD/KO Validated

SAP 97 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29292-1-PBS

Synonyme

DLG1, Disks large homolog 1, SAP-97, SAP97, hdlg

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  WB analysis using 29292-1-AP (same clone as 29292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29292-1-AP (DLG1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 29292-1-AP (same clone as 29292-1-PBS)

  WB result of SAP 97 antibody (29292-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAP 97 transfected HEK-293 cells. This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29292-1-AP (same clone as 29292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29292-1-AP (same clone as 29292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 29292-1-AP (same clone as 29292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 29292-1-AP (same clone as 29292-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SAP 97 antibody (29292-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29292-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SAP 97 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAP 97 fusion protein Ag31066
• Vollständiger Name: discs, large homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: NM_001098424
• Gene symbol: SAP97
• Gene ID (NCBI): 1739
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DLG1, also naemd as SAP97, hDlg and DLGH1, belongs to the MAGUK family. It is essential multidomain scaffolding protein required for normal development. DLG1 may play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. It regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. DLG1 is a functional regulator of Kv1.5 channel. The DLG1-MPP7 complex promotes epithelial cell polarity and tight junction formation. The antibody is specific to DLG1.