SAP 97 Polyklonaler Antikörper

SAP 97 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 55085-1-AP

Synonyme

Disks large homolog 1, DLG1, DLGH1, hdlg, SAP 97, SAP97, Synapse associated protein 97

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  WB analysis of mouse lung using 55085-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55085-1-AP (DLG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55085-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55085-1-AP (DLG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

55085-1-AP bindet in WB, IF, ELISA SAP 97 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: discs, large homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 130-140 kDa
• GenBank-Zugangsnummer: NM_004087
• Gene symbol: SAP97
• Gene ID (NCBI): 1739
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DLG1, also naemd as SAP97, hDlg and DLGH1, belongs to the MAGUK family. It is essential multidomain scaffolding protein required for normal development. DLG1 may play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. It regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. DLG1 is a functional regulator of Kv1.5 channel. The DLG1-MPP7 complex promotes epithelial cell polarity and tight junction formation. The antibody is specific to DLG1.

Publikationen

Species	Application	Title
mouse, human	IF	Kidney Int; Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney.; Authors - Rik Westland
rat	WB	Neurochem Res; PFOS Elicits Cytotoxicity in Neuron Through Astrocyte-Derived CaMKII-DLG1 Signaling In Vitro Rat Hippocampal Model; Authors - Jiawei Yang