SAP130 Polyklonaler Antikörper

SAP130 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12130-1-PBS

Synonyme

Sin3-associated polypeptide p130, 130 kDa Sin3-associated polypeptide

---

• 

  WB analysis of HEK-293 using 12130-1-AP (same clone as 12130-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12130-1-AP (same clone as 12130-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 12130-1-AP (same clone as 12130-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12130-1-AP (same clone as 12130-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

  IP result of anti-SAP130 (IP:12130-1-AP, 4ug; Detection:12130-1-AP 1:600) with HepG2 cells lysate 1000ug. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12130-1-AP (same clone as 12130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12130-1-AP (SAP130 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAP130 antibody (12130-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12130-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SAP130 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAP130 fusion protein Ag2774
• Vollständiger Name: Sin3A-associated protein, 130kDa
• Berechnetes Molekulargewicht: 110 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC017453
• Gene symbol: SAP130
• Gene ID (NCBI): 79595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SF3b is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAP 49, SAP 130, SAP 145, and SAP 155. SAP 130, SAP 145, and SAP 155 are present in a protein complex and associate with one another. While SAP 155 and SAP 130 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.