SAPS2 Polyklonaler Antikörper

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• KD/KO Validated

SAPS2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26190-1-AP

Synonyme

Serine/threonine-protein phosphatase 6 regulatory subunit 2, Serine/threonine protein phosphatase 6 regulatory subunit 2, SAPS domain family, member 2, SAPS domain family member 2, SAPS 2

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  WB analysis using 26190-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26190-1-AP (SAPS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26190-1-AP

  WB result of SAPS2 antibody (26190-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAPS2 transfected HepG2 cells.

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  IHC staining of human intrahepatic cholangiocarcinoma using 26190-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26190-1-AP (SAPS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 26190-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26190-1-AP (SAPS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26190-1-AP bindet in WB, IHC, ELISA SAPS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAPS2 fusion protein Ag23458
• Vollständiger Name: SAPS domain family, member 2
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 115-125 kDa
• GenBank-Zugangsnummer: BC000976
• Gene symbol: SAPS2
• Gene ID (NCBI): 9701
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAPS2 is a regulatory protein for the protein phosphatase-6 catalytic subunit. Together, these proteins act as a significant T-loop phosphatase for Aurora A, an essential mitotic kinase. Loss of function of either the regulatory or catalytic subunit of protein phosphatase-6 interferes with spindle formation and chromosome alignment.