SAPS3 Polyklonaler Antikörper

SAPS3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 16944-1-AP

Synonyme

SAPLa, SAPL, PPP6R3, PP6R3, KIAA1558

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  WB analysis using 16944-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16944-1-AP (SAPS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16944-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16944-1-AP (SAPS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 16944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16944-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16944-1-AP (SAPS3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

16944-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SAPS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAPS3 fusion protein Ag10555
• Vollständiger Name: SAPS domain family, member 3
• Berechnetes Molekulargewicht: 873 aa, 98 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC020953
• Gene symbol: SAPS3
• Gene ID (NCBI): 55291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; A conserved acetylation switch enables pharmacological control of tubby-like protein stability.; Authors - Evan M Kerek
human	IP	Cell Death Dis; PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death; Authors - Guowei Wu
	WB	BMC Biol; The protein phosphatase PP6 promotes RIPK1-dependent PANoptosis; Authors - Ratnakar R Bynigeri