SAR1A Rekombinanter Antikörper

SAR1A Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230471A1

Kat-Nr. : 83168-7-PBS

Synonyme

SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase, COPII associated small GTPase

---

• 

  IF Staining of HepG2 using 83168-7-RR (same clone as 83168-7-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SAR1A antibody (83168-7-RR, Clone: 230471A1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83168-7-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 83168-7-RR (same clone as 83168-7-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-7-RR, Clone:230471A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83168-7-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83168-7-RR (same clone as 83168-7-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-7-RR, Clone:230471A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83168-7-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

83168-7-PBS bindet in IF/ICC, ELISA SAR1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SAR1 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_020150
• Gene symbol: SAR1A
• Gene ID (NCBI): 56681
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).