SARDH Polyklonaler Antikörper

SARDH Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 22762-1-AP

Synonyme

BPR-2, EC:1.5.8.3, SAR, Sarcosine dehydrogenase, mitochondrial, SARD

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  WB analysis using 22762-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22762-1-AP (SARDH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 22762-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22762-1-AP (SARDH Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 22762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22762-1-AP (SARDH Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 22762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22762-1-AP (SARDH Antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

22762-1-AP bindet in WB, IHC, ELISA SARDH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARDH fusion protein Ag18738
• Vollständiger Name: sarcosine dehydrogenase
• Berechnetes Molekulargewicht: 918 aa, 101 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC136364
• Gene symbol: SARDH
• Gene ID (NCBI): 1757
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SARDH is a mitochondrial flavoenzyme that oxidizes sarcosine to glycine, feeding one-carbon units into folate metabolism. By tuning methyl-group balance, it modulates epigenetic programs and immune fate. Silencing or mutation of SARDH drives sarcosine accumulation, fueling invasion in prostate, liver, breast and kidney cancers, whereas its high expression predicts favorable prognosis. Thus SARDH acts as a metabolic tumor-suppressor and an emerging therapeutic target. (PMID: 23824605; 40830700; 31545450)

Publikationen

Species	Application	Title
human	WB	Cell Biochem Biophys; Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway; Authors - Long Miao
rat	WB	Sci Rep; Mutation in Smek2 regulating hepatic glucose metabolism causes hypersarcosinemia and hyperhomocysteinemia in rats; Authors - Yasutake Tanaka
mouse	WB	Brain Res; Brain transcriptomic, metabolic and mitohormesis properties associated with N-propargylglycine treatment: a prevention strategy against neurodegeneration; Authors - Fadzai Teramayi
human	IHC	J Cancer; Sarcosine dehydrogenase as an immune infiltration-associated biomarker for the prognosis of hepatocellular carcinoma; Authors - Haixiang Xie