SARM1 Polyklonaler Antikörper

SARM1 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 28625-1-AP

Synonyme

EC:3.2.2.-, EC:3.2.2.6, FLJ36296, hSARM1, HsTIR

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  WB analysis using 28625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 28625-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SARM1 antibody (28625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of SH-SY5Y using 28625-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SARM1 Polyclonal antibody (28625-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HEK-293-Zellen, humanes Hodengewebe, Maushirngewebe, Neuro-2a-Zellen, Raji-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

28625-1-AP bindet in WB, IF/ICC, FC (Intra), ELISA SARM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARM1 fusion protein Ag29531
• Vollständiger Name: sterile alpha and TIR motif containing 1
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: NM_015077
• Gene symbol: SARM1
• Gene ID (NCBI): 23098
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SARM1 (sterile alpha and Toll/interleukin-1 receptor motif-containing 1) is also named as KIAA0524, SAMD2 and SARM. SARM1 is the major pro-degenerative protein in axons (PMID: 32152523). SARM1 is the central executioner of pathological axon degeneration and is an inducible NAD+-cleavage enzyme that is activated by the loss of the NAD+ biosynthetic enzyme NMNAT2 (PMID: 33657413). SARM1 is involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PMID: 16964262).

Publikationen

Species	Application	Title
mouse	IF	Neurosci Res; SARM1 is essential for NMDA receptor-dependent endocytosis of AMPA receptors in hippocampal neurons; Authors - Misaki Morishita

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anam (Verified Customer) (09-03-2025)

For IF and ICC it works really well

• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Cell culture
• Primary Antibody Dilution: 1:200 or 1:1000
• Cell Tissue Type: Schwann cells, Stem cells