SARS Polyklonaler Antikörper

SARS Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 15162-1-AP

Synonyme

Seryl-tRNA(Ser/Sec) synthetase, Seryl-tRNA synthetase, SERS, SERRS, Serine--tRNA ligase, cytoplasmic

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  WB analysis of HEK-293 using 15162-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15162-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15162-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15162-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 15162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15162-1-AP (SARS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 15162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15162-1-AP (SARS antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15162-1-AP

  Immunofluorescent analysis of HepG2 cells using 15162-1-AP (SARS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15162-1-AP bindet in WB, IHC, IF/ICC, ELISA SARS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARS fusion protein Ag7270
• Vollständiger Name: seryl-tRNA synthetase
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58-64 kDa
• GenBank-Zugangsnummer: BC000716
• Gene symbol: SARS
• Gene ID (NCBI): 6301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SARS(Serine--tRNA ligase, cytoplasmic) Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).The deduced 514-amino acid protein has a calculated molecular mass of about 59 kD.And it can form a dimer of 120KDa(PMID:2180700).It is detected a band of 65KDa in MEL cell lines(PMID:1918033).

Publikationen

Species	Application	Title
human	WB	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu