SARS-COV-2 NSP5 Polyklonaler Antikörper

SARS-COV-2 NSP5 Polyklonal Antikörper für IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 29286-1-AP

Synonyme

SARS-COV-2 NSP5

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  IF Staining of HEK-293T using 29286-1-AP

  Immunofluorescent analysis of 4% PFA fixed HEK293T cells overexpressing ACE2 and TMPRSS using NSP5 antibody (29286-1-AP, green) at dilution of 1:200. Cells were infected with virus (dsRNA, in red, MOI 0.01, 48hpi). The images were credited by Dr. Irene Chen, Gladstone Institute.

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  ELISA experiment using 29286-1-AP

  SARS-CoV-2 Envelope protein Antibody (29286-1-AP) tested by ELISA. SARS-COV-2 NSP5 protein was coated onto microtiter plates at 1 µg/well and then incubated with a dilution series of SARS-COV-2 NSP5 protein Antibody (29286-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HEK-293T-Zellen
• Erfolgreiche Detektion in ELISA: N/A

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

29286-1-AP bindet in WB, IF/ICC, ELISA SARS-COV-2 NSP5 und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARS-COV-2 NSP5 fusion protein Ag30934
• Vollständiger Name: ORF1ab
• GenBank-Zugangsnummer: YP_009742612
• Gene symbol: SARS-COV-2 NSP8
• Gene ID (NCBI): 43740578
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Biol; SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12; Authors - Jinping Yang
	IF	Cell Rep; Pressure to evade cell-autonomous innate sensing reveals interplay between mitophagy, IFN signaling, and SARS-CoV-2 evolution; Authors - Jae Seung Lee
human	WB	J Biol Chem; E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MPro; Authors - Travis B Lear