SARS-CoV-2 Nucleocapsid Phosphoprotein Monoklonaler Antikörper

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoklonal Antikörper für WB, ELISA, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Virus

Anwendung

WB, ELISA, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3C3

Kat-Nr. : 67666-1-PBS

Synonyme

nucleocapsid phosphoprotein, 1B3C3, sars cov 2

---

• 

  WB analysis using 67666-1-Ig (same clone as 67666-1-PBS)

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP50061-1

  Sandwich ELISA standard curve of MP50061-1, Virus 2019-nCOV nucleocapsid phosphoprotein Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67666-1-PBS. Detection antibody: HRP-conjugated 67666-2-PBS. Standard: Ag30676. Range: 0.5-20 ng/mL.

• 

  ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

• 

  ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

  Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67666-1-PBS bindet in WB, ELISA, Sandwich ELISA, Indirect ELISA SARS-CoV-2 Nucleocapsid Phosphoprotein und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
• Vollständiger Name: COVID-19 N Protein
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: COVID-19 N Protein
• Gene ID (NCBI): 43740575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.