SARS-CoV-2 S protein (944-1214 aa) Monoklonaler Antikörper

SARS-CoV-2 S protein (944-1214 aa) Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Virus

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

CloneNo.

1H5E6

Kat-Nr. : 67794-1-Ig

Synonyme

S protein, SARS-CoV-2 Spike Glycoprotein, SARS-CoV-2 Spike Glycoprotein (944-1214 aa), spike, spike protein

---

Galerie der Validierungsdaten

• 

  WB analysis of Recombinant protein using 67794-1-Ig

  Spike Glycoprotein (944-1214 aa) were subjected to SDS PAGE followed by western blot with 67794-1-lg (SARS-CoV-2 Spike Glycoprotein (944-1214 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ag30682

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67794-1-Ig bindet in WB,ELISA SARS-CoV-2 S protein (944-1214 aa) und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SARS-CoV-2 S protein (944-1214 aa) fusion protein Ag30682
• Vollständiger Name: SARS-CoV-2 Spike Protein
• Berechnetes Molekulargewicht: 141 kDa
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: COVID-19 S Protein
• Gene ID (NCBI): 43740568
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).