SART3 Polyklonaler Antikörper

SART3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 18025-1-AP

Synonyme

DSAP1, hSART 3, KIAA0156, P100, p110

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  WB analysis of Jurkat using 18025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18025-1-AP (SART3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18025-1-AP (SART3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18025-1-AP

  IP result of anti-SART3 (IP:18025-1-AP, 4ug; Detection:18025-1-AP 1:700) with HeLa cells lysate 2000ug.

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  IHC staining of human testis using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18025-1-AP (SART3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18025-1-AP (SART3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Hirngewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe, Mausnierengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Produktinformation

18025-1-AP bindet in WB, IHC, IP, ELISA SART3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SART3 fusion protein Ag12590
• Vollständiger Name: squamous cell carcinoma antigen recognized by T cells 3
• Berechnetes Molekulargewicht: 963 aa, 110 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC093784
• Gene symbol: SART3
• Gene ID (NCBI): 9733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The process of mRNA splicing required assembly of pre-mRNA into a large, dynamic RNA-protein complex, in which 5 small nuclear RNAs and more than 50 protein components are incorporated into functional small nuclear ribonucleoproteins(snRNPs). SART3 interacts with U6 snRNA and functioned as a U6-specific recycling factor responsible for regeneration of base-paired U4/U6 snRNPs from post-spliceosomal free U4 and U6 snRNPs. Also it can interact and regulate Tat transactivation activity, and act as a cellular factor for HIV-1 gene expression and viral replication.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Enhanced packaging of U6 small nuclear RNA and splicing-related proteins into extracellular vesicles during HIV infection; Authors - Yiyao Huang
mouse,human	IP	RNA; 7SL RNA in Vertebrate Red Blood Cells.; Authors - Gaëlle J S Talhouarne
human	WB,IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen
		Mol Cell Biol; USP15 deubiquitinates TUT1 associated with RNA metabolism and maintains cerebellar homeostasis.; Authors - Jaehyun Kim
human	IHC	Aging (Albany NY); SART3, regulated by p53, is a biomarker for diagnosis, prognosis and immune infiltration in hepatocellular carcinoma; Authors - Jusen Nong