SASH1 Polyklonaler Antikörper

SASH1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27689-1-AP

Synonyme

KIAA0790, PEPE1, Proline-glutamate repeat-containing protein, RP3 323M4.1, SAM and SH3 domain-containing protein 1

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  WB analysis using 27689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27689-1-AP (SASH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 27689-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SASH1 antibody (27689-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, U-251-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27689-1-AP bindet in WB, IF/ICC, ELISA SASH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SASH1 fusion protein Ag26771
• Vollständiger Name: SAM and SH3 domain containing 1
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: NM_015278
• Gene symbol: SASH1
• Gene ID (NCBI): 23328
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAM and SH3 domain-containing protein 1 (SASH1, also known as KIAA0790 and PEPE1) is a multidomain protein implicated in various cellular processes, including cell adhesion, migration, and apoptosis. It has been identified as a novel Eph receptor-binding partner through SAM-SAM domain interactions, which are critical for the recruitment and binding of downstream molecules in the Eph signaling pathway (PMID: 37619706). SASH1 is also known to regulate signaling through focal adhesion kinase (FAK) and AKT/PI3K and promote apoptosis. Mutations in SASH1 have been associated with cancers, and it is suggested as a potential therapeutic target in non-small cell lung cancer (PMID: 33122723).