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SASH1 Polyklonaler Antikörper

SASH1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27689-1-PBS

Synonyme

KIAA0790, PEPE1, Proline-glutamate repeat-containing protein, RP3 323M4.1, SAM and SH3 domain-containing protein 1

Filter:
  • Western Blot (WB) analysis of various lysates using SASH1 Polyclonal antibody (27689-1-AP)

    WB analysis using 27689-1-AP (same clone as 27689-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27689-1-AP (SASH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27689-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SASH1 Polyclonal antibody (27689-1-AP)

    IF Staining of U2OS using 27689-1-AP (same clone as 27689-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SASH1 antibody (27689-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 27689-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27689-1-PBS bindet in WB, IF/ICC, Indirect ELISA SASH1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SASH1 fusion protein Ag26771
Vollständiger Name SAM and SH3 domain containing 1
Berechnetes Molekulargewicht 137 kDa
Beobachtetes Molekulargewicht170 kDa
GenBank-ZugangsnummerNM_015278
Gene symbol SASH1
Gene ID (NCBI) 23328
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAM and SH3 domain-containing protein 1 (SASH1, also known as KIAA0790 and PEPE1) is a multidomain protein implicated in various cellular processes, including cell adhesion, migration, and apoptosis. It has been identified as a novel Eph receptor-binding partner through SAM-SAM domain interactions, which are critical for the recruitment and binding of downstream molecules in the Eph signaling pathway (PMID: 37619706). SASH1 is also known to regulate signaling through focal adhesion kinase (FAK) and AKT/PI3K and promote apoptosis. Mutations in SASH1 have been associated with cancers, and it is suggested as a potential therapeutic target in non-small cell lung cancer (PMID: 33122723).