SAT1 Rekombinanter Antikörper

SAT1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240157G2

Kat-Nr. : 83319-1-PBS

Synonyme

240157G2, EC:2.3.1.57, Polyamine N-acetyltransferase 1, SAT, Spermidine/spermine N(1)-acetyltransferase 1

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  WB analysis using 83319-1-RR (same clone as 83319-1-PBS)

  Untreated and DENSPM treated HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 83319-1-RR (same clone as 83319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP00344-4

  The mean SAT1 concentration was determined to be 60.9 ng/mL in DENSPM-stimulated HeLa cell extracts based on a 3.0 mg/mL extract load.

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  Sandwich ELISA sample test of MP00344-4

  The mean SAT1 concentration was determined to be 62.4 ng/mL in U-251 cell extract based on a 1.4 mg/mL extract load.

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  Sandwich ELISA standard curve of MP00344-4

  Sandwich ELISA standard curve of MP00344-4, Human SAT1 Recombinant Matched Antibody Pair - PBS only. 83319-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1154. 83319-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  IHC staining of mouse brain using 83319-1-RR (same clone as 83319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83319-1-RR (SAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83319-1-RR (same clone as 83319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83319-1-RR (SAT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 83319-1-RR (same clone as 83319-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SAT1 antibody (83319-1-RR, Clone: 240157G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

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  FC experiment of RAW 264.7 using 83319-1-RR (same clone as 83319-1-PBS)

  1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug SAT1 Recombinant antibody (83319-1-RR, Clone:240157G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83319-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83319-1-PBS against Human SAT1 was performed. The affinity constant is 0.245 nM.

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  Affinity and Kinetic Characterization of 83319-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83319-1-RR against Human SAT1 were performed. The affinity constant is 0.484 nM.

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Geprüfte Anwendungen

Produktinformation

83319-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA SAT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SAT1 fusion protein Ag1154
• Vollständiger Name: spermidine/spermine N1-acetyltransferase 1
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 15-25 kDa
• GenBank-Zugangsnummer: BC008424
• Gene symbol: SAT1
• Gene ID (NCBI): 6303
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.