SATB1 Polyklonaler Antikörper

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• KD/KO Validated

SATB1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 15400-1-AP

Synonyme

Special AT-rich sequence-binding protein 1, DNA-binding protein SATB1

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  WB analysis of human brain using 15400-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15400-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15400-1-AP

  IP result of anti-SATB1 (IP:15400-1-AP, 4ug; Detection:15400-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human oesophagus cancer using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HEK-293-Zellen, Maushirngewebe, Mausmilzgewebe, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Urothelkarzinomgewebe, Mausmilzgewebe, Maus-Thymusgewebe, Rattenhirngewebe, Rattenmilzgewebe, Ratten-Thymusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

15400-1-AP bindet in WB, IHC, IP, CoIP, ChIP, ELISA SATB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SATB1 fusion protein Ag7615
• Vollständiger Name: SATB homeobox 1
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 86-100 kDa
• GenBank-Zugangsnummer: BC001744
• Gene symbol: SATB1
• Gene ID (NCBI): 6304
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epigenetic modifications and dynamic changes in chromatin organization by organizer proteins have recently been shown to play an instrumental role in regulating cancer-promoting genes. Special AT-rich binding protein (SATB1) is a unique type of global regulator that integrates higher-order chromatin organization -withregulation of gene expression. [PMID:23076250,22998183,23121661] SATB1 is a T cell-enriched transcription factor and a chromatin organizer essential for controlling genes that participate in T-cell development and activation. It regulates gene expression by periodically anchoring matrix attachment regions to the nuclear matrix and directly recruiting chromatin-modifying factors. Depending on its posttranslational modifications, SATB1 activates or represses multiple genes.Its expression is regulated by interleukin-4 (IL4) during T helper-2(Th2) cell differentiation[PMID: 20522714]. The calculated molecular weight of SATB1 is 86 kDa, but modified SATB1 is about 100 kDa (PMID: 22879953).

Publikationen

Species	Application	Title
		Dev Cell; Folding Keratin Gene Clusters during Skin Regional Specification.; Authors - Ya-Chen Liang
human	WB,IP	Cell Rep; SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites; Authors - Bao Wang ; KO Validated
mouse,rat	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
mouse	WB	Mol Neurobiol; SATB1/SLC7A11/HO-1 Axis Ameliorates Ferroptosis in Neuron Cells After Ischemic Stroke by Danhong Injection; Authors - Sikai Zhan
human	WB	Eur Rev Med Pharmacol Sci; Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.; Authors - X-L Zhang
human	WB	Int J Med Sci; SATB1 Knockdown Inhibits Proliferation and Invasion and Decreases Chemoradiation Resistance in Nasopharyngeal Carcinoma Cells by Reversing EMT and Suppressing MMP-9.; Authors - Dongni Zhou ; KD Validated