SATB2 Monoklonaler Antikörper

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• KD/KO Validated

SATB2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2F8E2

Rezensionen (1)

Kat-Nr. : 67958-1-Ig

Synonyme

KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2, DNA binding protein SATB2

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  WB analysis using 67958-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67958-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67958-1-Ig

  WB result of SATB2 antibody (67958-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SATB2 transfected HEK-293 cells.

• 

  WB analysis of fetal human brain using 67958-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW480 using 67958-1-Ig

  SW480 cells were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67958-1-Ig

  HepG2 cell lysates and HepG2 nuclear protein were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 67958-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67958-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67958-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, fetales humanes Hirngewebe, K-562-Zellen, NIH/3T3-Zellen, SW480-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67958-1-Ig bindet in WB, IHC, ELISA SATB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SATB2 fusion protein Ag17237
• Vollständiger Name: SATB homeobox 2
• Berechnetes Molekulargewicht: 733 aa, 83 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa
• GenBank-Zugangsnummer: BC098136
• Gene symbol: SATB2
• Gene ID (NCBI): 23314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SATB2, also named as KIAA1034, belongs to the CUT homeobox family. SATB2 binds to DNA at nuclear matrix- or scaffold-associated regions. STAB2 recognizes the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. SATB2 acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. It is a repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. SATB2 may play an important role in palate formation. SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. SATB2 has two isoforms produced by alternative splicing with the MW of 70 kDa and 83 kDa. It can be detected as 85-105 kDa by sumo modification (PMID: 14701874, PMID: 35140581).

Publikationen

Species	Application	Title
rat	WB	Ann Med; FOXG1 interaction with SATB2 promotes autophagy to alleviate neuroinflammation and mechanical abnormal pain in rats with lumbar disc herniation; Authors - Zhanchao Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

good Ab for cortical neurons

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cortical organoids