SBDS Monoklonaler Antikörper

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SBDS Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F8A10

Kat-Nr. : 67200-1-PBS

Synonyme

1F8A10, CGI 97, CGI-97, SDS, Shwachman-Bodian-Diamond syndrome protein

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  WB analysis using 67200-1-Ig (same clone as 67200-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67200-1-Ig (SBDS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67200-1-Ig (same clone as 67200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67200-1-Ig (SBDS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67200-1-Ig (same clone as 67200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67200-1-Ig (SBDS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67200-1-Ig (same clone as 67200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SBDS antibody (67200-1-Ig, Clone: 1F8A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67200-1-Ig (same clone as 67200-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SBDS antibody (67200-1-Ig, Clone: 1F8A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67200-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67200-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SBDS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SBDS fusion protein Ag11731
• Vollständiger Name: Shwachman-Bodian-Diamond syndrome
• Berechnetes Molekulargewicht: 250 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29-31 kDa
• GenBank-Zugangsnummer: BC065700
• Gene symbol: SBDS
• Gene ID (NCBI): 51119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined.