SBNO1 Polyklonaler Antikörper

SBNO1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25359-1-AP

Synonyme

Monocyte protein 3, MOP 3, MOP3, SBNO1, Sno

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  WB analysis using 25359-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25359-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25359-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25359-1-AP

  IP result of anti-SBNO1 (IP:25359-1-AP, 4ug; Detection:25359-1-AP 1:3000) with Jurkat cells lysate 1400 ug.

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  IF Staining of HeLa using 25359-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SBNO1 antibody (25359-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

25359-1-AP bindet in WB, IF/ICC, IP, ELISA SBNO1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SBNO1 fusion protein Ag19005
• Vollständiger Name: strawberry notch homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 1393 aa, 154 kDa
• Beobachtetes Molekulargewicht: 145-154 kDa
• GenBank-Zugangsnummer: BC133704
• Gene symbol: SBNO1
• Gene ID (NCBI): 55206
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SBNO1, also named as Monocyte protein 3 or MOP3, is a 1393 amino acid protein, which belongs to the SBNO family. SBNO1 play an important role in development of the vertebrate central nervous system. SBNO1 has four isoforms with MW 154 kDa and 80 kDa.

Publikationen

Species	Application	Title
mouse	WB,IF	Asian J Androl; Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice.; Authors - Cong Shen