SCAMP3 Polyklonaler Antikörper

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• KD/KO Validated

SCAMP3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (1)

Kat-Nr. : 26888-1-AP

Synonyme

C1orf3, PROPIN1, Secretory carrier-associated membrane protein 3

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  WB analysis using 26888-1-AP

  Huh7 cell lysates were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26888-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26888-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 26888-1-AP

  IP result of anti-SCAMP3 (IP:26888-1-AP, 4ug; Detection:26888-1-AP 1:5000) with HepG2 cells lysate 1480 ug.

• 

  IP experiment of mouse heart using 26888-1-AP

  IP result of anti-SCAMP3 (IP:26888-1-AP, 4ug; Detection:26888-1-AP 1:5000) with mouse heart tissue lysate 1880 ug.

• 

  IHC staining of mouse skeletal muscle using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HuH-7-Zellen, A549-Zellen, K-562-Zellen
• Erfolgreiche IP: HepG2-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

26888-1-AP bindet in WB, IHC, IF, IP, ELISA SCAMP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCAMP3 fusion protein Ag25256
• Vollständiger Name: secretory carrier membrane protein 3
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000161
• Gene symbol: SCAMP3
• Gene ID (NCBI): 10067
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Mol Cell; mTORC1 Activation Requires DRAM-1 by Facilitating Lysosomal Amino Acid Efflux.; Authors - Florian Beaumatin ; KD Validated
human	WB	Aging (Albany NY); Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.; Authors - Feiyu Mao
human	WB	Sci Rep; MicroRNA-27a/b-3p and PPARG regulate SCAMP3 through a feed-forward loop during adipogenesis.; Authors - Agné Kulyté
human	WB,IHC	Onco Targets Ther; SCAMP3 Promotes Glioma Proliferation and Indicates Unfavorable Prognosis via Multiple Pathways.; Authors - Chunliu Li ; KD Validated
human	WB	Onco Targets Ther; Bioinformatics Analysis and RNA-Sequencing of SCAMP3 Expression and Correlated Gene Regulation in Hepatocellular Carcinoma.; Authors - Shan-Shan Han
human	IF	Int J Biol Macromol; Fungal antitumor protein D1 is internalized via endocytosis and inhibits non-small cell lung cancer proliferation through MAPK signaling pathway; Authors - Min-Hui Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (02-15-2024)

This antibody provided some convincing IF staining but detected many unspecific bands when western blotting HUVEC lysate.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1 in 200 IF 1 in 1000 WB
• Cell Tissue Type: HUVEC