SCAMP5 Rekombinanter Antikörper

SCAMP5 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243122F5

Kat-Nr. : 85669-2-PBS

Synonyme

hSCAMP5, Secretory carrier-associated membrane protein 5

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  WB analysis using 85669-2-RR (same clone as 85669-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85669-2-RR (SCAMP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85669-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02041-2

  Cytometric bead array standard curve of MP02041-2, SCAMP5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85669-2-PBS. Detection antibody: 85669-1-PBS. Standard: Ag31837. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 85669-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85669-2-RR against Human SCAMP5 were performed. The affinity constant is 0.258 nM.

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Geprüfte Anwendungen

Produktinformation

85669-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA SCAMP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SCAMP5 fusion protein Ag31837
• Vollständiger Name: secretory carrier membrane protein 5
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC024700
• Gene symbol: SCAMP5
• Gene ID (NCBI): 192683
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Secretory carrier-associated membrane protein 5 (SCAMP5) belongs to the to the SCAMP family. Secretory carrier membrane proteins (SCAMPs) constitute a group of membrane transport proteins in plants, insects and mammals. The mammalian genome contains five types of SCAMP genes, namely, SCAMP1-SCAMP5. (PMID:36217917, PMID:19234194). SCAMPs participate in the vesicle cycling fusion of vesicles and cell membranes and play roles in regulating exocytosis and endocytosis, activating synaptic function and transmitting nerve signals. Among these proteins, SCAMP5 is highly expressed in the brain and has direct or indirect effects on the function of the central nervous system (PMID:36217917). SCAMP5 regulates membrane transport, controls the exocytosis of synaptic vesicles and is related to secretion carrier and membrane function. In addition, SCAMP5 plays a major role in the normal maintenance of the physiological functions of nerve cells (PMID:36217917, PMID:33663553). For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.