SCAP Polyklonaler Antikörper

SCAP Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 12266-1-AP

Synonyme

KIAA0199, SCAP, SREBF chaperone, SREBP cleavage-activating protein, Sterol regulatory element-binding protein cleavage-activating protein

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12266-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12266-1-AP (SCAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12266-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12266-1-AP (SCAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12266-1-AP (SCAP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 12266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12266-1-AP (SCAP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 12266-1-AP

  Immunofluorescent analysis of HEK-293 cells using 12266-1-AP (SCAP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

12266-1-AP bindet in WB, IHC, IF, ELISA SCAP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCAP fusion protein Ag2937
• Vollständiger Name: SREBF chaperone
• Berechnetes Molekulargewicht: 1279 aa, 140 kDa
• Beobachtetes Molekulargewicht: 140 kDa, 96 kDa
• GenBank-Zugangsnummer: BC020987
• Gene symbol: SCAP
• Gene ID (NCBI): 22937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCAP is a regulatory protein that is required for the proteolytic cleavage of the sterol regulatory element-binding protein (SREBP). SCAP is an integral membrane protein located in the endoplasmic reticulum (ER). One of the cytosolic regions of SCAP contains a hexapeptide amino acid sequence, MELADL, that functions to detect cellular cholesterol. When cholesterol is present, SCAP undergoes a conformational change that prevents it from activating SREBP and cholesterol synthesis does not occur. SCAP has 4 isoforms with MW 140, 98, 96 and 85 kDa (refer to UniProt: Q12770).

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; PMID: 24843127; Authors - Chong Wee Liew
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
human	WB	PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang
human	WB	bioRxiv; Identification of the novel role of sterol regulatory element binding proteins (SREBPs) in mechanotransduction and intraocular pressure regulation; Authors - Ting Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (08-14-2020)

Nice staining we got in our tissue sections

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human colon cancer tissue