SCAP Polyklonaler Antikörper

SCAP Polyklonal Antikörper für IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12266-1-PBS

Synonyme

SREBP cleavage-activating protein, SREBP cleavage activating protein, SREBFchaperone, SREBF chaperone, PSEC0227

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  IP experiment of HEK-293 using 12266-1-AP (same clone as 12266-1-PBS)

  IP result of anti-SCAP (IP:12266-1-AP, 4ug; Detection:12266-1-AP 1:600) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 12266-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 12266-1-AP (same clone as 12266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12266-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 12266-1-AP (same clone as 12266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12266-1-AP (SCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12266-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12266-1-PBS bindet in IHC, IP, Indirect ELISA SCAP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCAP fusion protein Ag2937
• Vollständiger Name: SREBF chaperone
• Berechnetes Molekulargewicht: 1279 aa, 140 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC020987
• Gene symbol: SCAP
• Gene ID (NCBI): 22937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCAP is a regulatory protein that is required for the proteolytic cleavage of the sterol regulatory element-binding protein (SREBP). SCAP is an integral membrane protein located in the endoplasmic reticulum (ER). One of the cytosolic regions of SCAP contains a hexapeptide amino acid sequence, MELADL, that functions to detect cellular cholesterol. When cholesterol is present, SCAP undergoes a conformational change that prevents it from activating SREBP and cholesterol synthesis does not occur. SCAP has 4 isoforms with MW 140, 98, 96 and 85 kDa (refer to UniProt: Q12770).