SCD Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SCD Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

36

Kat-Nr. : 23393-1-AP

Synonyme

Acyl CoA desaturase, Delta(9) desaturase, FADS5, Fatty acid desaturase, MSTP008, SCD, SCD1, SCDOS, Stearoyl CoA desaturase

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Galerie der Validierungsdaten

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  IHC staining of human liver using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 23393-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23393-1-AP (SCD Antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• IHC: See 10 publications below
• IF: See 2 publications below

Produktinformation

23393-1-AP bindet in IHC, IF, ELISA SCD und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCD fusion protein Ag13456
• Vollständiger Name: stearoyl-CoA desaturase (delta-9-desaturase)
• Berechnetes Molekulargewicht: 355 aa, 41 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC005807
• Gene symbol: SCD
• Gene ID (NCBI): 6319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCD (stearoyl-CoA desaturase) is a 37-45 kDa (PMID:10946019) microsomal fatty acid monodesaturase, also commonly known as 9-desaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids. Five SCD genes (SCD1, SCD2, SCD3,SCD4 and SCD5) have been identified and characterized in mice and SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively, SCD3 is expressed exclusively in skin, whereas SCD4 is expressed predominantly in the heart. SCD1and SCD5 has been the only human SCD gene identified and characterized todate(PMID:15907797 ). The formation of homodimers and oligomers is an intrinsic property of SCD proteins, which may play an important role in regulating the half-life of the SCDenzymes, thus representing a novel regulatory mechanism for SCD enzymes, in addition to the transcriptional and post-translational regulations. A 32-kDa SCD1 degradation product identified may be caused by a major cleavage site at the C-terminus, thus representing a novel degradation product(PMID:15610069).

Publikationen

Species	Application	Title
human		Nucleic Acids Res; EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma.; Authors - Xianping Shi
human		EMBO J; A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.; Authors - Doowon Huh
human,mouse	IHC	Stem Cells; Increased lipogenesis is critical for self-renewal and growth of breast cancer stem cells: Impact of omega-3 fatty acids.; Authors - Haiqing Luo ; KD Validated
human		Liver Int; Disruption of Plin5 degradation by CMA causes lipid homeostasis imbalance in NAFLD.; Authors - Shuo Y Ma
mouse		J Physiol; Sex-specific metabolic responses to 6 hours of fasting during the active phase in young mice.; Authors - Therese Freire
human		Int J Biol Sci; Long noncoding RNA SH3PXD2A-AS1 promotes colorectal cancer progression by regulating p53-mediated gene transcription.; Authors - Pingfu Hou