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SCD1 Polyklonaler Antikörper
SCD1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF/ICC, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 28678-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A431-Zellen, A375-Zellen, A549-Zellen, HepG2-Zellen | 
| Erfolgreiche Detektion in IHC | Mauslebergewebe,  humanes Kolonkarzinomgewebe,  humanes Lebergewebe,  humanes Ovarialkarzinomgewebe,  humanes Magenkrebsgewebe,  braunes Mausfettgewebe,  Rattenherzgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | MCF-7-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 | 
| Immunhistochemie (IHC) | IHC : 1:50-1:500 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 2 publications below | 
| WB | See 46 publications below | 
| IHC | See 6 publications below | 
| IF | See 2 publications below | 
| CoIP | See 1 publications below | 
Produktinformation
28678-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA SCD1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Affe, Maus, Ratte, Ziege | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | SCD1 fusion protein Ag29156 | 
| Vollständiger Name | stearoyl-CoA desaturase (delta-9-desaturase) | 
| Berechnetes Molekulargewicht | 355 aa, 41 kDa | 
| Beobachtetes Molekulargewicht | 28-42 kDa | 
| GenBank-Zugangsnummer | BC005807 | 
| Gene symbol | SCD | 
| Gene ID (NCBI) | 6319 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for SCD1 antibody 28678-1-AP | Protokoll herunterladen | 
| IHC protocol for SCD1 antibody 28678-1-AP | Protokoll herunterladenl | 
| IF protocol for SCD1 antibody 28678-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Adv Sci (Weinh) Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells | ||
| ACS Appl Mater Interfaces Lactate-Fueled Theranostic Nanoplatforms for Enhanced MRI-Guided Ferroptosis Synergistic with Immunotherapy of Hepatocellular Carcinoma | ||
| Phytomedicine Melittin suppresses ovarian cancer growth by regulating SREBP1-mediated lipid metabolism | ||
| Free Radic Biol Med Zinc deficiency drives ferroptosis resistance by lactate production in esophageal squamous cell carcinoma | ||
| Inflammation Multiple Machine Learning Identifies Key Gene PHLDA1 Suppressing NAFLD Progression | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH YINGJIAN (Verified Customer) (08-07-2025) | The antibody demonstrates high specificity and strong affinity toward the target protein. Besides, the shipment very quick. It is overnight shipment. 
  | 
| FH Marco (Verified Customer) (07-09-2024) | It does its job in NRVCMs 
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