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SCD1 Polyklonaler Antikörper

SCD1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(50)

Kat-Nr. : 28678-1-AP

Synonyme

SCD, EC:1.14.19.1, Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, A375-Zellen, A549-Zellen, HepG2-Zellen
Erfolgreiche Detektion in IHCMauslebergewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Ovarialkarzinomgewebe, humanes Magenkrebsgewebe, braunes Mausfettgewebe, Rattenherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:16000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

28678-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA SCD1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Maus, Ratte, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SCD1 fusion protein Ag29156
Vollständiger Name stearoyl-CoA desaturase (delta-9-desaturase)
Berechnetes Molekulargewicht 355 aa, 41 kDa
Beobachtetes Molekulargewicht28-42 kDa
GenBank-ZugangsnummerBC005807
Gene symbol SCD
Gene ID (NCBI) 6319
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SCD1 antibody 28678-1-APProtokoll herunterladen
IHC protocol for SCD1 antibody 28678-1-APProtokoll herunterladenl
IF protocol for SCD1 antibody 28678-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nature

7-Dehydrocholesterol dictates ferroptosis sensitivity

Authors - Yaxu Li
human,mouseWB,IHC

Adv Sci (Weinh)

Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells

Authors - Ling Li
mouseWB

ACS Appl Mater Interfaces

Lactate-Fueled Theranostic Nanoplatforms for Enhanced MRI-Guided Ferroptosis Synergistic with Immunotherapy of Hepatocellular Carcinoma

Authors - Xiaoxiao Chen
mouseWB

Phytomedicine

Melittin suppresses ovarian cancer growth by regulating SREBP1-mediated lipid metabolism

Authors - Ruixin Wu
humanWB

Free Radic Biol Med

Zinc deficiency drives ferroptosis resistance by lactate production in esophageal squamous cell carcinoma

Authors - Peiyan Yang
human,mouseWB

Inflammation

Multiple Machine Learning Identifies Key Gene PHLDA1 Suppressing NAFLD Progression

Authors - Zhenwei Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

YINGJIAN (Verified Customer) (08-07-2025)

The antibody demonstrates high specificity and strong affinity toward the target protein. Besides, the shipment very quick. It is overnight shipment.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: liver
SCD1 Antibody Western Blot validation (1:5000 dilution) in liver (Cat no:28678-1-AP)
FH

Marco (Verified Customer) (07-09-2024)

It does its job in NRVCMs

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: NRVCMs