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SCF Polyklonaler Antikörper

SCF Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23844-1-AP

Synonyme

KITLG, c Kit ligand, c-Kit ligand, KIT ligand, Kitl

Filter:
  • Western Blot (WB) analysis of various lysates using SCF Polyclonal antibody (23844-1-AP)

    WB analysis using 23844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23844-1-AP (SCF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SCF Polyclonal antibody (23844-1-AP)

    IHC staining of mouse kidney using 23844-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23844-1-AP (SCF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SCF Polyclonal antibody (23844-1-AP)

    IHC staining of mouse kidney using 23844-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23844-1-AP (SCF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SCF Polyclonal antibody (23844-1-AP)

    IF Staining of HepG2 using 23844-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SCF Polyclonal antibody (23844-1-AP)

    IF Staining of HEK-293 using 23844-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBK-562-Zellen
Erfolgreiche Detektion in IHCMausnierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

23844-1-AP bindet in WB, IHC, IF/ICC, ELISA SCF und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SCF fusion protein Ag20837
Vollständiger Name KIT ligand
Berechnetes Molekulargewicht 273 aa, 31 kDa
Beobachtetes Molekulargewicht 31 kDa
GenBank-ZugangsnummerBC074725
Gene symbol SCF
Gene ID (NCBI) 4254
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-affinitätsgereinigt
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stem cell factor (SCF) is also named as mast cell growth factor (MGF), Kit ligand (Kitl), c-Kit ligand, steel factor and Kitlg. SCF is a pleiotropic cytokine (also as a dimeric molecule) that mediates regulatory effects on inflammation, tissue remodeling, and fibrosis through binding to and activating its receptor tyrosine kinase c-Kit (PMID: 1698553, PMID: 8630386, PMID: 23073628). SCF is the ligand of c-Kit, a member of the RTKs family. Molecular activity of RTKs is a primary stimulus of cell motility, and expression of the SCF/c-Kit axis is associated with cell migration (PMID: 28629522). SCF is a cytokine that synergizes the effects of Epo during erythropoiesis. Stem cell factor plays a well-described role in regulating the survival, proliferation, migration, and differentiation of hematopoietic progenitors, melanocytes, and germ cells (PMID: 36688460). More recent studies have demonstrated SCF expression in dermal and intestinal epithelial cells, and increased SCF expression has been reported in the inflamed mucosa of individuals with IBD and colonitis (PMID: 18200049, PMID: 30157426).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SCF antibody 23844-1-APProtokoll herunterladen
IHC protocol for SCF antibody 23844-1-APProtokoll herunterladenl
IF protocol for SCF antibody 23844-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Udesh (Verified Customer) (08-12-2025)

Worked well for western in MSCs, showed a non specific band at 70 kDa, but also detected the protein.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MSCs