SCF Polyklonaler Antikörper

SCF Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23844-1-PBS

Synonyme

KITLG, c Kit ligand, c-Kit ligand, KIT ligand, Kitl

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  WB analysis using 23844-1-AP (same clone as 23844-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23844-1-AP (SCF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23844-1-AP (same clone as 23844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23844-1-AP (SCF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23844-1-AP (same clone as 23844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23844-1-AP (SCF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 23844-1-AP (same clone as 23844-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 23844-1-AP (same clone as 23844-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23844-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SCF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCF fusion protein Ag20837
• Vollständiger Name: KIT ligand
• Berechnetes Molekulargewicht: 273 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC074725
• Gene symbol: SCF
• Gene ID (NCBI): 4254
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stem cell factor (SCF) is also named as mast cell growth factor (MGF), Kit ligand (Kitl), c-Kit ligand, steel factor and Kitlg. SCF is a pleiotropic cytokine (also as a dimeric molecule) that mediates regulatory effects on inflammation, tissue remodeling, and fibrosis through binding to and activating its receptor tyrosine kinase c-Kit (PMID: 1698553, PMID: 8630386, PMID: 23073628). SCF is the ligand of c-Kit, a member of the RTKs family. Molecular activity of RTKs is a primary stimulus of cell motility, and expression of the SCF/c-Kit axis is associated with cell migration (PMID: 28629522). SCF is a cytokine that synergizes the effects of Epo during erythropoiesis. Stem cell factor plays a well-described role in regulating the survival, proliferation, migration, and differentiation of hematopoietic progenitors, melanocytes, and germ cells (PMID: 36688460). More recent studies have demonstrated SCF expression in dermal and intestinal epithelial cells, and increased SCF expression has been reported in the inflamed mucosa of individuals with IBD and colonitis (PMID: 18200049, PMID: 30157426).