SCF Rekombinanter Antikörper

SCF Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240787H1

Kat-Nr. : 83707-2-RR

Synonyme

Kitlg, Mgf, Kitl, KIT ligand, 240787H1

---

• 

  WB analysis using 83707-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 83707-2-RR

  PC-12 cells were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83707-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83707-2-RR against Rat SCF were performed. The affinity constant is 0.624 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Neuro-2a-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83707-2-RR bindet in WB, ELISA SCF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: KIT ligand
• Berechnetes Molekulargewicht: 31kDa
• Beobachtetes Molekulargewicht: 28 kDa, 45 kDa
• GenBank-Zugangsnummer: NM_021843.4
• Gene symbol: Kitlg
• Gene ID (NCBI): 60427
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis. SCF is a 35 to 45 kDa monomeric glycoprotein.