Secretogranin V Polyklonaler Antikörper

Secretogranin V Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 10761-1-AP

Synonyme

SCG5, Secretogranin-5, N-terminal peptide, Neuroendocrine protein 7B2, C-terminal peptide

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  WB analysis of mouse brain using 10761-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10761-1-AP (Secretogranin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat pancreas using 10761-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat pancreas using 10761-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary adenoma using 10761-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary adenoma using 10761-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 10761-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-12 cells using 10761-1-AP (Secretogranin V antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Ratten-Pankreasgewebe, humanes Hypophysenadenom-Gewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10761-1-AP bindet in WB, IHC, IF/ICC, ELISA Secretogranin V und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Secretogranin V fusion protein Ag1107
• Vollständiger Name: secretogranin V (7B2 protein)
• Berechnetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC005349
• Gene symbol: Secretogranin V
• Gene ID (NCBI): 6447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma.; Authors - Takashi Sakaguchi
mouse	WB	Aging (Albany NY); Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin; Authors - Yuqi Xin
human	WB,IHC	Dis Esophagus; Expression profile and function of secretogranin V, and its effects on the malignant behavior of esophageal squamous cell carcinoma; Authors - Mohammad Hussain Hamrah ; KD Validated
mouse	IF	Diabetologia; Transcriptomic heterogeneity of non-beta islet cells is associated with type 2 diabetes development in mouse models; Authors - Pascal Gottmann