Nav1.5 Polyklonaler Antikörper

Nav1.5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 23016-1-AP

Synonyme

CDCD2, CMD1E, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SCN5A, SSS1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausherzgewebe
Erfolgreiche Detektion in IHCMaus-Skelettmuskelgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

23016-1-AP bindet in WB, IHC, IF, ELISA Nav1.5 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Nav1.5 fusion protein Ag19275
Vollständiger Name sodium channel, voltage-gated, type V, alpha subunit
Berechnetes Molekulargewicht 2016 aa, 227 kDa
Beobachtetes Molekulargewicht 227 kDa
GenBank-ZugangsnummerBC140813
Gene symbol SCN5A
Gene ID (NCBI) 6331
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Nav1.5 antibody 23016-1-APProtokoll herunterladen
IHC protocol for Nav1.5 antibody 23016-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Cancer Lett

Sialyltransferase ST3GAL4 confers osimertinib resistance and offers strategies to overcome resistance in non-small cell lung cancer

Authors - Rui Han
human

Front Pharmacol

Spider venom-derived peptide JZTX-14 prevents migration and invasion of breast cancer cells via inhibition of sodium channels

Authors - Wenfang Wu
mouseWB

Int Immunopharmacol

Salidroside treatment decreases the susceptibility of atrial fibrillation in diabetic mice by reducing mTOR-STAT3-MCP-1 signaling and atrial inflammation

Authors - Wenpu Ren
ratWB

Hum Mol Genet

Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy.

Authors - Kuang-Yung Lee
ratWB,IF

Eur J Pharmacol

Glucose fluctuations aggravated the late sodium current induced ventricular arrhythmias via the activation of ROS/CaMKII pathway

Authors - Feng Li
mouseWB

Biochim Biophys Acta Mol Basis Dis

Changes in ion channel expression and function associated with cardiac arrhythmogenic remodeling by Sorbs2.

Authors - Ling-Ling Qian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Udesh (Verified Customer) (01-19-2023)

Used at 1:500 dilution in 5% BSA and probed overnight at 4 degrees C. Upper membrane piece shows Nav1.5 bands close to 150 kD. Multiple bands seen probably due to BSA.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
Nav1.5 Antibody Western Blot validation (1:500 dilution) in (Cat no:23016-1-AP)
FH

Rebecca (Verified Customer) (03-16-2022)

10ug of protein was loaded. Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK-293T
Nav1.5 Antibody Western Blot validation (1:5000 dilution) in HEK-293T (Cat no:23016-1-AP)