SCN9A/Nav1.7-Specific Polyklonaler Antikörper

SCN9A/Nav1.7-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 20257-1-AP

Synonyme

Nav1.7, SCN9A, NENA, NE NA, hNE-Na

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  WB analysis using 20257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20257-1-AP (SCN9A/Nav1.7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20257-1-AP (SCN9A/Nav1.7-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20257-1-AP (SCN9A/Nav1.7-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Hausschwein-Hirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 6 publications below

Produktinformation

20257-1-AP bindet in WB, IHC, IF, ELISA SCN9A/Nav1.7-Specific und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: sodium channel, voltage-gated, type IX, alpha subunit
• Berechnetes Molekulargewicht: 226 kDa
• Beobachtetes Molekulargewicht: 226 kDa
• GenBank-Zugangsnummer: NM_002977
• Gene symbol: SCN9A
• Gene ID (NCBI): 6335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCN9A, also named as NENA, PN1, ETHA, NE-NA, Nav1.7 and hNE-Na, belongs to the sodium channel family. SCN9A mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, SCN9A forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na+ channel isoform. SCN9a plays a role in pain mechanisms, especially in the development of inflammatory pain. Defects in SCN9A are the cause of primary erythermalgia or autosomal recessive congenital indifference to pain or paroxysmal extreme pain disorder (PEPD). The antibody is specific to SCN9A

Publikationen

Species	Application	Title
mouse	IF	Prog Neurobiol; MicroRNA-96 is required to prevent allodynia by repressing voltage-gated sodium channels in spinal cord.; Authors - Liting Sun
rat	WB,IF	J Neuroinflammation; Glial interleukin-1β upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats.; Authors - Peng Zhang
rat	WB	Inflammation; Prostaglandin E2 Upregulated Trigeminal Ganglionic Sodium Channel 1.7 Involving Temporomandibular Joint Inflammatory Pain in Rats.; Authors - Peng Zhang
rat	WB	Chin J Dent Res; Non-steroidal Anti-inflammatory Drugs Attenuate Hyperalgesia and Block Upregulation of Trigeminal Ganglionic Sodium Channel 1.7 after Induction of Temporomandibular Joint Inflammation in Rats.; Authors - Rui Yun Bi
rat	WB,IF	Neurosci Lett; Connexin 43 contributes to temporomandibular joint inflammation induced-hypernociception via sodium channel 1.7 in trigeminal ganglion.; Authors - Yi-Zhou Jin
mouse	IF	Front Mol Neurosci; Ectopic expression of Nav1.7 in spinal dorsal horn neurons induced by NGF contributes to neuropathic pain in a mouse spinal cord injury model; Authors - Yan Fu