SCO1 Polyklonaler Antikörper

SCO1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12614-1-PBS

Synonyme

SCOD1

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  WB analysis using 12614-1-AP (same clone as 12614-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 12614-1-AP (same clone as 12614-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12614-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 12614-1-AP (same clone as 12614-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 12614-1-AP (same clone as 12614-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 12614-1-AP (same clone as 12614-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12614-1-AP (SCO1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12614-1-PBS bindet in WB, IHC, ELISA SCO1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCO1 fusion protein Ag3269
• Vollständiger Name: SCO cytochrome oxidase deficient homolog 1 (yeast)
• Berechnetes Molekulargewicht: 301 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC015504
• Gene symbol: SCO1
• Gene ID (NCBI): 6341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human SCO1 and SCO2 are metallochaperones that are essential for the assembly of the catalytic core of cytochrome c oxidase (COX). SCO1 appears to be involved in the folding and assembly of cytochrome oxidase subunit II, and possibly of other proteins of this subcompartment. It is a 33 kDa protein which displays limited homology to members of the DnaJ family(PMID:14731768). It is also named as SCOD1 and belongs to the SCO1/2 family. It can exsit as a homodimer(PMID:16735468). Defects in SCO1 are a cause of mitochondrial complex IV deficiency (MT-C4D).