SCP2/SCPx Polyklonaler Antikörper

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• KD/KO Validated

SCP2/SCPx Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23006-1-PBS

Synonyme

SCP2, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16, EC:2.3.1.176

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  WB analysis using 23006-1-AP (same clone as 23006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23006-1-AP (same clone as 23006-1-PBS)

  WB result of SCP2 antibody (23006-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 23006-1-AP (same clone as 23006-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 23006-1-AP (same clone as 23006-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

  IP result of anti-SCP2 (IP:23006-1-AP, 4ug; Detection:23006-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23006-1-AP (SCP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SCP2 (23006-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23006-1-PBS bindet in WB, IF/ICC, FC (Intra), IP, Indirect ELISA SCP2/SCPx und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCP2/SCPx fusion protein Ag19215
• Vollständiger Name: sterol carrier protein 2
• Berechnetes Molekulargewicht: 547 aa, 59 kDa
• Beobachtetes Molekulargewicht: 13-15 kDa
• GenBank-Zugangsnummer: BC005911
• Gene symbol: SCP2
• Gene ID (NCBI): 6342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 23006-1-AP antibody recognizes the 13-15 kDa SCP2 protein.