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SCP2/SCPx Rekombinanter Antikörper

SCP2/SCPx Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250813C8

Kat-Nr. : 86196-3-PBS

Synonyme

SCP2, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16, EC:2.3.1.176

Filter:
  • Western Blot (WB) analysis of various lysates using SCP2/SCPx Recombinant antibody (86196-3-RR)

    WB analysis using 86196-3-RR (same clone as 86196-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86196-3-RR (SCPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of k562 using SCP2/SCPx Recombinant antibody (86196-3-RR)

    IF Staining of k562 using 86196-3-RR (same clone as 86196-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed k562 using SCP2/SCPx antibody (86196-3-RR, Clone: 250813C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86196-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86196-3-RR

    Affinity and Kinetic Characterization of 86196-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86196-3-RR against Human SCP2/SCPx were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

86196-3-PBS bindet in WB, IF/ICC, Indirect ELISA SCP2/SCPx und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen SCP2/SCPx fusion protein Ag5895
Vollständiger Name sterol carrier protein 2
Berechnetes Molekulargewicht 59 kDa
Beobachtetes Molekulargewicht46 kDa, 58 kDa
GenBank-ZugangsnummerBC067108
Gene symbol SCP2
Gene ID (NCBI) 6342
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. It recognizes the intact 58 kDa SCPx protein as well as 44-46 kDa thiolase (PMID: 35996156).