SCP2/SCPx Rekombinanter Antikörper

SCP2/SCPx Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250813C8

Kat-Nr. : 86196-3-RR

Synonyme

SCP2, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16, EC:2.3.1.176

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  WB analysis using 86196-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86196-3-RR (SCPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of k562 using 86196-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed k562 using SCP2/SCPx antibody (86196-3-RR, Clone: 250813C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86196-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86196-3-RR against Human SCP2/SCPx were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HepG2-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, U-87 MG-Zellen
• Erfolgreiche Detektion in IF/ICC: K562

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86196-3-RR bindet in WB, IF/ICC, ELISA SCP2/SCPx und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SCP2/SCPx fusion protein Ag5895
• Vollständiger Name: sterol carrier protein 2
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 58 kDa
• GenBank-Zugangsnummer: BC067108
• Gene symbol: SCP2
• Gene ID (NCBI): 6342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. It recognizes the intact 58 kDa SCPx protein as well as 44-46 kDa thiolase (PMID: 35996156).