SCPEP1 Rekombinanter Antikörper

SCPEP1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240886B6

Kat-Nr. : 83786-4-PBS

Synonyme

RISC, Retinoid-inducible serine carboxypeptidase, MSTP034, HSCP1, EC:3.4.16.-

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  WB analysis of U-251 using 83786-4-RR (same clone as 83786-4-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83786-4-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 83786-4-RR (same clone as 83786-4-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83786-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83786-4-RR (same clone as 83786-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83786-4-RR (SCPEP1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83786-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83786-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83786-4-RR against Human SCPEP1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83786-4-PBS bindet in WB, IHC, ELISA SCPEP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SCPEP1 fusion protein Ag31381
• Vollständiger Name: serine carboxypeptidase 1
• Berechnetes Molekulargewicht: 452 aa, 51 kDa
• Beobachtetes Molekulargewicht: 33 kDa, 45~51 kDa
• GenBank-Zugangsnummer: BC072405
• Gene symbol: SCPEP1
• Gene ID (NCBI): 59342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364).