SCPX Rekombinanter Antikörper

SCPX Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250813B3

Kat-Nr. : 86196-1-PBS

Synonyme

SCP2, SCP2/SCPx, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16

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  WB analysis using 86196-1-RR (same clone as 86196-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 86196-1-RR (same clone as 86196-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 86196-1-RR (same clone as 86196-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 86196-1-RR (same clone as 86196-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 86196-1-RR (same clone as 86196-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 86196-1-RR (same clone as 86196-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02291-1

  Sandwich ELISA standard curve of MP02291-1, Human SCP2/SCPx Recombinant Matched Antibody Pair - PBS only. 86196-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5895. 86196-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

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  Sample test of MP02291-1

  The mean SCP2/SCPx concentration was determined to be 109.35 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load and 125.11 ng/mL in HepG2 cell extract based on a 1.10 mg/mL extract load.

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  Sample test of MP02291-1

  The mean SCP2/SCPx concentration was determined to be 249.0 ng/mL in HepG2 cell extract based on a 0.9 mg/mL extract load.

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  IF Staining of HEK-293 using 86196-1-RR (same clone as 86196-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCPX antibody (86196-1-RR, Clone: 250813B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02291-1

  Cytometric bead array standard curve of MP02291-1, SCP2/SCPx Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86196-2-PBS. Detection antibody: 86196-1-PBS. Standard: Ag5895. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86196-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86196-1-RR against Human SCPX were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

86196-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test SCPX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SCPX fusion protein Ag5895
• Vollständiger Name: sterol carrier protein 2
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 58 kDa
• GenBank-Zugangsnummer: BC067108
• Gene symbol: SCP2
• Gene ID (NCBI): 6342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCPX protein is a kind of protein related to cholesterol absorption and transport, belonging to SCP-2 gene family. SCPX protein contains an N-terminal thiolase domain and a C-terminal SCP-2 domain. In cells, SCPX protein will be cut into 44-46 kDa thiolase and 13-15 kDa SCP-2 protein. SCPX-2 is a nonspecific lipid transporter, which is involved in the transport and metabolism of intracellular lipids. It is distributed in peroxisome, cytoplasm and nucleus, and may play a role as a transcription factor in nucleus.