SCPX Rekombinanter Antikörper

SCPX Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250813B3

Kat-Nr. : 86196-1-RR

Synonyme

SCP2, SCP2/SCPx, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16

---

• 

  WB analysis using 86196-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 86196-1-RR

  rat liver tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 86196-1-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 86196-1-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 86196-1-RR

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 86196-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 86196-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCPX antibody (86196-1-RR, Clone: 250813B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 86196-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86196-1-RR against Human SCPX were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, HepG2-Zellen, K-562-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, U-87 MG-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86196-1-RR bindet in WB, IF/ICC, ELISA SCPX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SCPX fusion protein Ag5895
• Vollständiger Name: sterol carrier protein 2
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 58 kDa
• GenBank-Zugangsnummer: BC067108
• Gene symbol: SCP2
• Gene ID (NCBI): 6342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCPX protein is a kind of protein related to cholesterol absorption and transport, belonging to SCP-2 gene family. SCPX protein contains an N-terminal thiolase domain and a C-terminal SCP-2 domain. In cells, SCPX protein will be cut into 44-46 kDa thiolase and 13-15 kDa SCP-2 protein. SCPX-2 is a nonspecific lipid transporter, which is involved in the transport and metabolism of intracellular lipids. It is distributed in peroxisome, cytoplasm and nucleus, and may play a role as a transcription factor in nucleus.