SCTR Rekombinanter Antikörper

SCTR Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240675E6

Kat-Nr. : 83675-6-RR

Synonyme

SR, secretin receptor, SCT-R, SCT R, 240675E6

---

• 

  WB analysis using 83675-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83675-6-RR (SCTR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of THP-1 using 83675-6-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SCTR antibody (83675-6-RR, Clone: 240675E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 83675-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83675-6-RR against Human SCTR were performed. The affinity constant is 6.05 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83675-6-RR bindet in WB, IF/ICC, ELISA SCTR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SCTR fusion protein Ag5371
• Vollständiger Name: SCTR
• Berechnetes Molekulargewicht: 440 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC035757
• Gene symbol: SCTR
• Gene ID (NCBI): 6344
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCTR is a member of the family B G protein-coupled receptors. SCTR is a receptor for SCT, a gastrointestinal peptide hormone secreted by the S cells of the duodenum. SCT regulates water homeostasis throughout the body and influences the environment of the duodenum by regulating SCT in the stomach and pancreas. Studies suggest that SCT can act as a neuropeptide within the central nervous system (CNS), thus SCTR may regulate the function of the CNS.