SDCCAG8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SDCCAG8 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 13471-1-AP

Synonyme

NPHP10, HSPC085, hCCCAP, CCCAP, BBS16

---

• 

  IHC staining of mouse brain using 13471-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13471-1-AP (SDCCAG8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13471-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13471-1-AP (SDCCAG8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13471-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SDCCAG8 antibody (13471-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Produktinformation

13471-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SDCCAG8 und zeigt Reaktivität mit human, Hund, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Hund, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SDCCAG8 fusion protein Ag4264
• Vollständiger Name: serologically defined colon cancer antigen 8
• Berechnetes Molekulargewicht: 713 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC032454
• Gene symbol: SDCCAG8
• Gene ID (NCBI): 10806
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC. SDCCAG8 has 4 isoforms with MW 83,78,73 and 41 kDa (refer to UniProt). Catalog#13471-1-AP can recognize all the four isoforms.

Publikationen

Species	Application	Title
human	WB	Nature; Global landscape of HIV-human protein complexes.; Authors - Stefanie Jäger
human, zebrafish	WB,IF	Nat Genet; Candidate exome capture identifies mutation of SDCCAG8 as the cause of a retinal-renal ciliopathy.; Authors - Otto Edgar A EA
mouse	WB, IF, IP	Neuron; SDCCAG8 Regulates Pericentriolar Material Recruitment and Neuronal Migration in the Developing Cortex.; Authors - Ryan Insolera
mouse	WB, IHC, IF	J Am Soc Nephrol; Renal-retinal ciliopathy gene sdccag8 regulates DNA damage response signaling.; Authors - Rannar Airik ; KD Validated
human	IF	J Cell Biol; Centrobin controls primary ciliogenesis in vertebrates.; Authors - Yetunde Adesanya Ogungbenro
mouse	WB, IF	Cell Death Dis; Selective loss of RPGRIP1-dependent ciliary targeting of NPHP4, RPGR and SDCCAG8 underlies the degeneration of photoreceptor neurons.; Authors - Patil H H