SDHA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SDHA Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

86

Rezensionen (1)

Kat-Nr. : 14865-1-AP

Synonyme

FP, SDH2, SDHA, SDHF

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Galerie der Validierungsdaten

• 

  WB analysis using 14865-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14865-1-AP (SDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14865-1-AP

  IP result of anti-SDHA(IP:14865-1-AP, 4ug; Detection:14865-1-AP 1:20000) with HEK-293 cells lysate 960 ug.

• 

  IHC staining of human liver cancer using 14865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14865-1-AP (SDHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14865-1-AP (SDHA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14865-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SDHA antibody (14865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 80 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

14865-1-AP bindet in WB, IP, IHC, IF, ELISA SDHA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SDHA fusion protein Ag6677
• Vollständiger Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 62-73 kDa
• GenBank-Zugangsnummer: BC001380
• Gene symbol: SDHA
• Gene ID (NCBI): 6389
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Publikationen

Species	Application	Title
human	WB	Science; Fumarate is a terminal electron acceptor in the mammalian electron transport chain.; Authors - Jessica B Spinelli ; KO Validated
mouse	WB	Cell Metab; The requirement for pyruvate dehydrogenase in leukemogenesis depends on cell lineage.; Authors - Sojeong Jun
human	WB	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	Mol Cell; Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery; Authors - Jasmin Adriana Schafer
mouse	WB	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jessica (Verified Customer) (08-19-2019)

This antibody worked great for western blots on whole cell lysates and purified mitochondria!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Osteosarcoma cell line

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