SDHA Monoklonaler Antikörper

SDHA Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5E10G12

Kat-Nr. : 66588-1-PBS

Synonyme

5E10G12, EC:1.3.5.1, FP, MC2DN1, PGL5

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  WB analysis using 66588-1-Ig (same clone as 66588-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66588-1-Ig (SDHA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66588-1-Ig (same clone as 66588-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SDHA antibody (66588-1-Ig, Clone: 5E10G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50790-1

  Cytometric bead array standard curve of MP50790-1, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-2-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

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  Cytometric bead array standard curve of MP50790-2

  Cytometric bead array standard curve of MP50790-2, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-3-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

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  Cytometric bead array standard curve of MP50790-3

  Cytometric bead array standard curve of MP50790-3, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-4-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66588-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA SDHA und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SDHA fusion protein Ag7023
• Vollständiger Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 62-73 kDa
• GenBank-Zugangsnummer: BC001380
• Gene symbol: SDHA
• Gene ID (NCBI): 6389
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.