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SDHD Polyklonaler Antikörper

SDHD Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26519-1-AP

Synonyme

PGL, CybS, CII-4, CII4, CII 4

Filter:
  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SDHD Polyclonal antibody (26519-1-AP)

    IHC staining of human stomach cancer using 26519-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26519-1-AP (SDHD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SDHD Polyclonal antibody (26519-1-AP)

    IF Staining of HepG2 using 26519-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SDHD antibody (26519-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in IHChumanes Magenkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

26519-1-AP bindet in IHC, IF/ICC, ELISA SDHD und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SDHD fusion protein Ag24644
Vollständiger Name succinate dehydrogenase complex, subunit D, integral membrane protein
Berechnetes Molekulargewicht 17 kDa
GenBank-ZugangsnummerBC012603
Gene symbol SDHD
Gene ID (NCBI) 6392
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IHC protocol for SDHD antibody 26519-1-APProtokoll herunterladenl
IF protocol for SDHD antibody 26519-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Lana (Verified Customer) (09-07-2020)

Antibody detects multiple bands as well as specific 17kDa SDHD band on WB.SDS-PAGE: 15 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:1000.Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 and 3 – mitochondrial fraction lysates.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse embryonic stem cells
SDHD Antibody Western Blot validation (1:1000 dilution) in Mouse embryonic stem cells (Cat no:26519-1-AP)