SEC13 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SEC13 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Rezensionen (1)

Kat-Nr. : 15397-1-AP

Synonyme

D3S1231E, GATOR2 complex protein SEC13, Protein SEC13 homolog, SEC13A, SEC13L1

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  WB analysis using 15397-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15397-1-AP (SEC13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15397-1-AP

  IP result of anti-SEC13 (IP:15397-1-AP, 4ug; Detection:15397-1-AP 1:10000) with HepG2 cells lysate 1760 ug.

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  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 15397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SEC13 antibody (15397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 15397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using SEC13 antibody (15397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HepG2 using 15397-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC13 antibody (15397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, L02-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IF: See 1 publications below

Produktinformation

15397-1-AP bindet in WB, IHC, IF/ICC, IF-Fro, IP, ELISA SEC13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC13 fusion protein Ag7610
• Vollständiger Name: SEC13 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC002634
• Gene symbol: SEC13
• Gene ID (NCBI): 6396
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)

Publikationen

Species	Application	Title
human	WB	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
human	WB	Nat Commun; Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation; Authors - Xing Chen
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
mouse,human	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
rat	WB	Proc Natl Acad Sci U S A; TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER-Golgi intermediate compartments.; Authors - Michael G Hanna
human	WB	Proc Natl Acad Sci U S A; Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.; Authors - Liling Niu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Subir (Verified Customer) (09-27-2019)

clean and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from mouse tail tendon

SEC13 Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:15397-1-AP)