SEC16A Polyklonaler Antikörper

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• KD/KO Validated

SEC16A Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20025-1-PBS

Synonyme

SEC16L, SEC16 homolog A, SEC16, RP11 413M3.10, p250

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  WB analysis of MCF-7 using 20025-1-AP (same clone as 20025-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20025-1-AP (SEC16A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

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  WB analysis using 20025-1-AP (same clone as 20025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20025-1-AP (SEC16A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 20025-1-AP (same clone as 20025-1-PBS)

  IP result of anti-SEC16A (IP:20025-1-AP, 4ug; Detection:20025-1-AP 1:2000) with HeLa cells lysate 840 ug. This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 20025-1-AP (same clone as 20025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 20025-1-AP (same clone as 20025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 20025-1-AP (same clone as 20025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 20025-1-AP (same clone as 20025-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SEC16A antibody (20025-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 20025-1-AP (same clone as 20025-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SEC16A (20025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20025-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA SEC16A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SEC16 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 234 kDa
• Beobachtetes Molekulargewicht: 250-300 kDa
• GenBank-Zugangsnummer: NM_014866
• Gene symbol: SEC16A
• Gene ID (NCBI): 9919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.