SEC22B Polyklonaler Antikörper

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• KD/KO Validated

SEC22B Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14776-1-AP

Synonyme

ER Golgi SNARE of 24 kDa, ERS 24, ERS24, SEC22B, SEC22L1

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Galerie der Validierungsdaten

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  WB analysis using 14776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14776-1-AP (SEC22B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14776-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14776-1-AP (SEC22B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14776-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14776-1-AP (SEC22B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14776-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14776-1-AP (SEC22B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14776-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14776-1-AP (SEC22B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14776-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14776-1-AP (SEC22B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14776-1-AP (SEC22B antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 14776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14776-1-AP (SEC22B antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 14776-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14776-1-AP (SEC22B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

14776-1-AP bindet in WB, IHC, IF, ELISA SEC22B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC22B fusion protein Ag6077
• Vollständiger Name: SEC22 vesicle trafficking protein homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC001364
• Gene symbol: SEC22B
• Gene ID (NCBI): 9554
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins are critical components of the membrane fusion machinery (PMID: 20163564). SEC22B is a SNARE involved in endoplasmic reticulum (ER)/Golgi membrane trafficking. SEC22B localizes to the ER-Golgi intermediate compartment (ERGIC) and has been identified as a regulator of antigen crosspresentation (PMID: 22153078).

Publikationen

Species	Application	Title
human	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
mouse	WB,IF	Nat Cell Biol; The SNARE Sec22b has a non-fusogenic function in plasma membrane expansion.; Authors - Maja Petkovic
		Sci Adv; Integrated proteogenetic analysis reveals the landscape of a mitochondrial-autophagosome synapse during PARK2-dependent mitophagy.; Authors - Jin-Mi Heo
mouse	WB	J Neurosci; Unbalanced regulation of Sec22b and Ykt6 blocks autophagosome axonal retrograde flux in neuronal ischemia-reperfusion injury.; Authors - Haiying Li ; KD Validated
rat	WB,IF	Brain Res Bull; Upregulation of Sec22b plays a neuroprotective role in a rat model of traumatic brain injury via inducing protective autophagy.; Authors - Di Li ; KD Validated

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