SEC22B Rekombinanter Antikörper

SEC22B Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242659B3

Kat-Nr. : 85037-5-RR

Synonyme

242659B3, ER-Golgi SNARE of 24 kDa, ERS24, ERS-24, SEC22 vesicle-trafficking protein homolog B

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  WB analysis using 85037-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85037-5-RR (SEC22B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 85037-5-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SEC22B Recombinant antibody (85037-5-RR, Clone:242659B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85037-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85037-5-RR against Human SEC22B were performed. The affinity constant is 2.07 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, CHO-Zellen, fetales humanes Hirngewebe, humanes Plazenta-Gewebe, MCF-7-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85037-5-RR bindet in WB, FC (Intra), ELISA SEC22B und zeigt Reaktivität mit hamster, human, Maus, Ratten

• Getestete Reaktivität: hamster, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SEC22B fusion protein Ag12981
• Vollständiger Name: SEC22 vesicle trafficking protein homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC001364
• Gene symbol: SEC22B
• Gene ID (NCBI): 9554
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins are critical components of the membrane fusion machinery (PMID: 20163564). SEC22B is a SNARE involved in endoplasmic reticulum (ER)/Golgi membrane trafficking. SEC22B localizes to the ER-Golgi intermediate compartment (ERGIC) and has been identified as a regulator of antigen crosspresentation (PMID: 22153078).