SEC23IP Polyklonaler Antikörper

SEC23IP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20892-1-PBS

Synonyme

---

• 

  WB analysis using 20892-1-AP (same clone as 20892-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 20892-1-AP (same clone as 20892-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 20892-1-AP (same clone as 20892-1-PBS)

  IP result of anti-SEC23IP (IP:20892-1-AP, 4ug; Detection:20892-1-AP 1:1000) with HeLa cells lysate 3600ug. This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20892-1-AP (same clone as 20892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20892-1-AP (same clone as 20892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 20892-1-AP (same clone as 20892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 20892-1-AP (same clone as 20892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20892-1-AP (same clone as 20892-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

20892-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SEC23IP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC23IP fusion protein Ag14916
• Vollständiger Name: SEC23 interacting protein
• Berechnetes Molekulargewicht: 1000 aa, 111 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: BC063800
• Gene symbol: SEC23IP
• Gene ID (NCBI): 11196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC23-interacting protein (SEC23IP), also known as p125, is a member of the phosphatidic acid preferring-phospholipase A1 family. SEC23IP interacts with Sec23, a coat component of COPII vesicles which are involved in protein export from the endoplasmic reticulum (ER). SEC23IP localizes to ER exit sites and participates in the organization of this compartment (PMID: 15623529). It may have a role in the early stage of the secretory pathway (PMID: 10400679).